Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645284 | Gene | 2018 | 25 Pages |
Abstract
As a key member of the cytochrome P450 gene superfamily, CYP17 gene encodes 17α-hydroxylase/C17,20-lyase that is critical for directing androgen synthesis. The CYP17 gene has been identified in several species, yet little is known about its distribution and expression profile during goose follicular development. In the present study, we obtained the full-length coding sequence of goose CYP17 (gCYP17) gene for the first time using RACE method. Its sequence alignment and phylogenetic analysis suggested that gCYP17 was highly conserved with those of other birds and consisted of four main functional domains like other species. Results from immunohistochemistry, quantitative real-time PCR and Western blotting suggested that gCYP17 was predominantly located in theca interna throughout follicular development. Furthermore, levels of gCYP17 reached the maximum in theca layer of the 6-8â¯mm follicles which were significantly higher than in those of other follicles (Pâ¯<â¯0.05). In addition, gCYP17 was expressed at much higher levels in the F4 theca layer than the F1 follicle (Pâ¯<â¯0.05). Therefore, these results indicated that the fluctuating expression pattern and specific cellular localization of gCYP17 during follicular development might be closely related to androgen secretion, and thereby follicular maturation.
Keywords
SWF17α-hydroxylase/17,20-lyaseDABCYP17 geneHRPCYP173,3′-diaminobenzidineq-PCRamino acidrapid amplification of cDNA endscoding sequencefollicular developmentgranulosa cellGooseCellular localizationRacefollicle stimulating hormoneluteinizing hormoneFSHquantitative real-time PCRHorseradish peroxidaseCdSAndrogen Receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Huilan Huang, Da Chen, Shenqiang Hu, Jiwen Wang, Hehe Liu, Jiwei Hu, Liang Li,