Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645393 | Gene | 2018 | 32 Pages |
Abstract
Nasopharyngeal carcinoma (NPC) is a head and neck cancer associated with poor prognosis. Many studies have shown that the epithelial-to-mesenchymal transition (EMT) is important in cancer progression, metastasis, and chemotherapy resistance and that microRNAs (miRNAs) play a key role in chemotherapy resistance associated with EMT. The miRNA miR-139-5p is downregulated in many human cancers and is closely related to tumor progression. The aim of this study was to investigate the ability of miR-139-5p to influence the cisplatin resistance, apoptosis, invasion and migration in NPC cells through the regulation of the EMT. We investigated these processes in parental HNE1 and cisplatin-resistant HNE1/DDP cells transfected with miR-139-5p inhibitors and mimics, respectively. Our results suggest that the upregulation of miR-139-5p expression inhibits proliferation, invasion, migration and EMT in human NPC cells. In addition, we found that miR-139-5p expression levels and DDP-induced apoptosis positively correlate in NPC cells. In conclusion, our results demonstrate that miR-139-5p can regulate the migration, invasion and DDP resistance in human NPC by modulating the EMT. The regulation of miR-139-5p levels might be a new approach to reverse EMT and DDP resistance and counteract metastasis and chemotherapy resistance in human NPC.
Keywords
MMP-9P glycoproteinTPBSZEBDDPBcl-2 associated XP-gpNPCDAPIBcl-2HRPGAPDHIC50PVDFmiRNAsPBSSDSPI3KFBSqPCR4′,6-diamidino-2-phenylindoleMAPKQuantitative PCRsmall nuclear RNAAktSDS-polyacrylamide gel electrophoresisSDS-PAGEBaxEMTstandard error of the meanpolyvinylidene difluoridemicroRNAssodium dodecyl sulphateNSCLCNon-small cell lung cancerfetal bovine serumcisplatinphosphoinositide 3-kinaseB-cell lymphoma 2matrix metallopeptidasePhosphate-buffered salineSEMDrug-resistancehalf maximal inhibitory concentrationHorseradish peroxidaseprotein kinase Bmitogen-activated protein kinasePropidium iodideNasopharyngeal carcinomanegative controlepithelial-to-mesenchymal transition
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Authors
Qianqian Shao, Pei Zhang, Yingye Ma, Zhaoyi Lu, Jie Meng, Hui Li, Xiaoming Wang, Deshang Chen, Mingjie Zhang, Yaofeng Han, Hao Liu, Shiyin Ma,