Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645407 | Gene | 2018 | 10 Pages |
Abstract
Color polymorphism is an ecologically important trait, which is related to local adaptation and ecological speciation. The pea aphid Acyrthosiphon pisum shows color polymorphism: the red and green color morphs where differences in ecological adaptation have been observed. Here, we measured genome-wide gene expression profiles of two color morphs in natural populations of A. pisum to explore the genetic basis of differentiated ecological adaptation. The results showed that only 32 genes were significantly differentially expressed between the two morphs, of which 18 had functional annotations. Among them, 13 genes were up-regulated [e.g. genes encoding protoheme IX farnesyltransferase (LOC100570971), carotene dehydrogenase (tor) and V-type proton ATPase subunit B (LOC100169462)] and 5 genes were down-regulated in the red morph (e.g. genes encoding transcription factors and heat shock proteins). To assess the functional importance of these differentially expressed genes (DEGs), we selected three highly expressed DEGs (LOC100169462, LOC100570971 and tor) with functional annotations and analyzed their expression levels in the red morph under three low temperatures (1â¯Â°C, 4â¯Â°C, and 8â¯Â°C) for 24â¯h. These three DEGs showed an interesting expression response to the cold acclimating conditions which resulted in an obvious phenotypic change of the red individuals to be greenish variants. This study suggests a link between gene expressions and body color polymorphisms in the pea aphid and provides important clues for further studying molecular mechanisms of ecological adaptation in aphids.
Keywords
qRT-PCRNCBI non-redundant protein sequencesHSPsDEGsAphidsTORMRSAMethicillin resistant Staphylococcus aureusGene expressionCold stimulationKEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and GenomesEcological adaptationGene ontologyquantitative real-time PCRHeat shock proteinsColor polymorphismDifferentially expressed genes
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Genetics
Authors
Li Zhang, Meng-Yao Wang, Xiao-Peng Li, Xiao-Tong Wang, Cheng-Lin Jia, Xing-Zhuo Yang, Run-Qiu Feng, Ming-Long Yuan,