Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645568 | Gene | 2018 | 9 Pages |
Abstract
Porcine butyrophilin-like 5 (Btnl5) is a novel member of the butyrophilin family, which consists of immune regulators. The expression pattern and the function of this gene remain unclear. In this study, Btnl5 is identified as a negative regulator of the NF-κB pathway. Our study indicates that Btnl5 is mainly expressed in intestinal epithelial cells (IECs) and expressed in membrane systems. Btnl5 inhibits MyD88-mediated activation of the NF-κB pathway. Btnl5 interacts with TNF receptor-associated factor 2 (TRAF2) and transcription factor p65. Besides, Btnl5 inhibits p65-mediated activation of the NF-κB pathway and inhibits nuclear translocation of p65. These results suggest that Btnl5 may inhibit NF-κB pathway through binding and interfere nuclear translocation of p65.
Keywords
NF-κBBTNRIP1TRAF2βgalIκBDAPITRIM25IPTGPVDF4′,6-diamidino-2-phenylindoleEDTAethylene diamine tetraacetic acidβ-galactosidasebutyrophilinInteracting proteinsrapid-amplification of cDNA endsIntestinal epithelial cellsTNF receptor-associated factor 2RaceImmune regulationinhibitory κBPolyvinylidene fluorideIECs
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Authors
Qi Bao, Chong Li, Chongfeng Xu, Ruiying Zhang, Kai Zhao, Ziyuan Duan,