Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8645809 | Gene | 2018 | 9 Pages |
Abstract
Mesenchymal stem cells (MSC) from bone marrow hold great potential as a cell source for cartilage repair. The objective of our study was differentiation of MSC toward chondrocyte by using Nanofiber-based polyethersulfone (PES) scaffold and also enhanced chondrogenic differentiation of BMSC in vitro. MSCs were harvested from bone marrow of human and PES scaffold was fabricated via Electrospinning. The isolated cells were cultured on the PES scaffold and scaffold free method. After 21Â days, Real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. Also, in order to confirm our results, we have done immunocytochemistry and SEM imaging. Flowcytometry confirmed the nature of the isolated adherent cells. Immunocytochemistry and SEM imaging confirmed the differentiation of MSC toward chondrocyte. Also, real time PCR showed a significant increased gene expression of collagen type II and aggrecan on the PES scaffold method when compared to the mRNA levels measured in scaffold free method. Down regulation of Collagen type I was observed in PES scaffold compared to scaffold free at day 21. Also, both methods showed a similar pattern of expression of SOX9. Our results showed that PES scaffold maintains BMSC proliferation and differentiation, and can significantly enhance chondrogenic differentiation of BMSC. PES scaffold seeded BMSC showed the highest capacity for differentiation into chondrocyte-like cells.
Keywords
GAPDHCOL1A1BMSCCol2a1collagen type XMSCFGFCol10a1PESTCPSTGF-βFBSDMEMPBSDulbecco's modified Eagle MediumOsteoarthritistransforming growth factor-βfetal bovine serumBone marrow mesenchymal stem cellMesenchymal stem cellsfibroblast growth factorphosphate-buffered saline (PBS)SEMBMPscanning electron microscopebone morphogenetic proteinspolyethersulfoneTissue culture polystyreneCollagen type Icollagen type IIChondrocytesglyceraldehyde-3-phosphate dehydrogenase
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Genetics
Authors
Hossein Mahboudi, Bahram Kazemi, Masoud Soleimani, Hana Hanaee-Ahvaz, Hossein Ghanbarian, Mojgan Bandehpour, Seyed Ehsan Enderami, Mousa Kehtari, Ghasem Barati,