Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8646045 | Gene | 2018 | 8 Pages |
Abstract
Mitogen-activated protein kinase (MAPK) signaling pathway plays an important role in cellular response to inflammatory cytokines, environmental stress and pathogenic infection, However, compared with mammals and insects, the potential function of p38 MAPK from Chinese shrimp (Fenneropenaeus chinensis) in response to viruses and bacterial infection is limited. In the study, the immune responses of four genes MKK3, MKK4, p38 and ATF-2 from F. chinensis were investigated in defending against white spot syndrome virus (WSSV), Vibrio anguillarum (V. anguillarum) and Staphylococcus aureus (S. aureus) infection. Results demonstrated that the expression levels of these four genes were apparently modulated in hemocytes and gills when shrimp were stimulated by WSSV or bacteria, particularly at 3-24Â h after infection. MKK3, p38 and ATF-2 were most sensitive to V. anguillarum (Gram-negative bacteria), followed by WSSV and S. aureus (Gram-positive bacteria), while MKK4 gene was most sensitive to S. aureus, followed by WSSV and V. anguillarum. Knockdown of Fcp38 by RNA interference (RNAi) resulted in a reduction in the expression of FcMKK3 and FcATF-2. The results indicate that p38MAPK signaling pathway plays a role in defending against viral and bacterial infections in F. chinensis.
Keywords
p38mitogen-activated protein kinase kinase 3MKK6V. anguillarumFenneropenaeus chinensisMKK3ATF-2mitogen-activated protein kinase kinase 4MKK4Vibrio anguillarumWSSVRT-PCRERKsMAPKRNA interferenceRNAiStaphylococcus aureusS. aureusBacteriaANOVAone-way analysis of varianceRNA interference (RNAi)reverse transcription PCRactivating transcription factor 2White spot syndrome virusmitogen-activated protein kinaseExpression profilesextracellular signal-regulated kinases
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Authors
Yuying He, Wanlong Yao, Ping Liu, Jian Li, Qingyin Wang,