Association of the G/T rs4646 of CYP19 gene polymorphism with oxidative stress, vitamin A and estradiol in Iraqi women with endometriosis disease

This study is designed to investigate the relationship between oxidative stress and single nucleotide polymorphism G/T rs4646 of CYP19 and determine the main effects of SNP on the gene expression in Iraqi women with endometriosis disease. Data were collected from sixty women, mean age (31.16 ± 5.61) years as referred by a Gynecologist for endometriosis investigation. A total of thirty healthy female volunteers served as control, mean age (31.96 ± 5.42) years. The results revealed a highly significant increased (P < 0.01) in the malondialdehyde (MDA). The results of the Coenzyme Q10 vitamin A, β-carotene and vitamin E showed a highly significant decreased (P < 0.01) in the sera levels of women with endometriosis as compared to control. The results showed a significant (P ≤ 0.01) association between oxidative stress and selected SNP of CYP19 gene. No significant differences (P > 0.05) were observed in the level of LH, FSH, and Testosterone among different genotypes, while a significant increase (P < 0.05) in the level of E2 in women have TT genotype. A highly significant difference (P ≤ 0.01) in CYP19 gene expression from women with versus without endometriosis and increased by 3.82-fold in women with endometriosis. In this regard, we suggest that decreased of antioxidant vitamins and increased of MDA have an effect on the production of ovarian steroid hormones, in particular CYP19 gene, which encodes aromatase that is the key enzyme for estrogen biosynthesis. We deduce that CYP19 gene polymorphism is associated with the presence of endometriosis and can be considered a potential biomarker of the disease. Our findings may lead to new insights into the manner of development of endometriosis disease. The association between SNP G/T rs4646 of CYP19 and MDA suggest that SNP may play important role in the ovarian antioxidant-oxidant balance.