Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8646214 | Gene Reports | 2018 | 31 Pages |
Abstract
Retrograde transport of VEGF was confirmed for detection of pVEGFR1/VEGFR2 in the DRG. PMM surgery led to development of OA and mechanical allodynia, with reduced paw withdrawal thresholds (PWT) (Pâ¯<â¯0.0001). IT injection of MF-1 led to a reduction of allodynia in advanced OA, but injection of DC101 did not. IA injection of MF-1 or DC101 at one week after PMM injury did not reduce allodynia, but when injected in advanced OA mice joints at 12â¯weeks, both Mabs increased PWT an indicator of analgesia. Our data show that MF-1 (VEGR1 inhibition) decreases pain in advanced OA after IT or IA injection. Activation of MF-1 or DC101 may ameliorate OA-related joint pain.
Keywords
mAbsWntPDGFNGFPWTECMHBSSPIGFVEGFR2RT-PCRSASPPTTCX43VEGFR1IACUCDRGFDAFlk-1Flt-1PMMdorsal root ganglionHanks balanced salt solutionpaw withdrawal thresholdProbabilityFood & Drug AdministrationOsteoarthritisUnited States of AmericaUSAintrathecalstandard error of the meanPainInstitutional Animal Care and Use CommitteeHourHeparin sulfateplacental growth factorVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)platelet-derived growth factornerve growth factorSenescence-associated secretory phenotypeExtracellular matrixSEMIntraarticularreal-time polymerase chain reactionMonoclonal antibodies
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Authors
Vaskar Das, Ranjan Kc, Xin Li, InSug O-Sullivan, Andre J. van Wijnen, Jeffrey S. Kroin, Bronislaw Pytowski, Daniel T. Applegate, Gina Votta-Velis, Richard L. Ripper, Thomas J. Park, Hee-Jeong Im,