| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8646262 | Gene Reports | 2018 | 12 Pages |
Abstract
We observed variation in stability of reference gene expression with different stimuli and between human retinal endothelial cell lines. Our findings support adherence to MIQE recommendations regarding normalization in RT-qPCR studies of human retinal endothelial cells.
Keywords
b2mIL-1βSOCS1PGK1Rps18PUM1UBCsdhAPPIADMOGdimethyloxalylglycineribosomal protein S18HMBSLDHAALAS1HPRT1HBBTFRCDSCR1YWHAZRPLP0GUSBglucuronidase betaRPL30FBSDMEMubiquitin CTBPG6PDribosomal protein L13AGAPDHICAM-1RT-qPCRVEGFALPSRPL13ARibosomal protein L30Dulbecco's modified Eagle's mediumactin betaHumaninterleukin 1βBeta-2-microglobulintumor necrosis factor αMIQEfetal bovine serumsuppressor of cytokine signaling 1Endothelial cellRetinaActbVascular endothelial growth factorTNF-αPhosphoglycerate kinase 1Lactate dehydrogenase Alipopolysaccharidecyclin-dependent kinase inhibitor 1AIntercellular adhesion molecule 1nonohydroxymethylbilane synthaseHypoxanthine phosphoribosyltransferase 1PCRReference genetransferrin receptor
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Authors
Binoy Appukuttan, Liam M. Ashander, Yuefang Ma, Justine R. Smith,