Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8648412 | Molecular Immunology | 2018 | 8 Pages |
Abstract
The discovery of heat shock protein 16â¯kDa antigen protein has deepen the understanding of latent tuberculosis since it was found to be primarily expressed by Mycobacterium tuberculosis during latent phase leading to the rapid optimization and development in terms of diagnosis and therapeutics. Recently, T cell receptor-like antibody has been explored extensively targeting various diseases due to its dual functionality (T cell receptor and antibody). In this study, a TCR-like domain antibody (A2/Ab) with the binding capacity to Mtb heat shock protein (HSP) 16â¯kDa antigen presented by major histocompatible complex (MHC) HLA-A*02 was successfully generated via biopanning against human domain antibody library. The generated antibody (A2/Ab) exhibited strong functionality and binding capacity against the target assuring the findings of this study to be beneficial for the development of latent tuberculosis diagnosis and immunotherapeutics in future.
Keywords
ABTSIEDBTCr2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium saltscFvIPTGHspAPCMTBAPDβ2-mTregssdAbPBSDABfragment variableBSAregulatory T-cellsbovine serum albuminSingle domain antibodyHuman leukocyte antigenAntigen presenting cellsHLAenzyme-linked immunosorbent assaysUltravioletimmunosurveillanceimmunotherapyBeta 2-microglobulinTuberculosisELISAvariable heavy chainWorld Health OrganizationLatent tuberculosisAntibody phage displayphosphate buffer salineMycobacterium tuberculosismajor histocompatibility complexMHCHeat shock proteinpolyethylene glycolPEGWHOT cell receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Molecular Biology
Authors
Sylvia Annabel Dass, Mohd Nor Norazmi, Armando Acosta Dominguez, Maria Elena Sarmiento Garcia San Miguel, Gee Jun Tye,