Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8648450 | Molecular Immunology | 2018 | 10 Pages |
Abstract
Granulocytes and natural killer (NK) cells have been linked to brain injury in ischemic stroke. However, their recruitment from peripheral leucocytes in stroke patients is not well understood. Here, the expression of the interleukin 8 (CXCL8) in plasma, and CXC chemokine receptor 2 (CXCR2) in peripheral leucocytes of patients with ischemic stroke were evaluated. Based on the results, CXCR2 expression positively correlated with granulocytes and NK cells, which were in turn attracted by CXCL8. The results also indicated that CXCR2 was a direct target of microRNA (miR)-4437, a negative regulator of CXCR2, which was downregulated in peripheral leucocytes from patients with ischemic stroke. Furthermore, serum CXCL8 levels were associated with the infarct volume and functional outcomes in patients with ischemic stroke. The results of the receiver operating characteristic curve analysis with an optimal cut-off value of 34âpg/mL indicated serum CXCL8 levels could be a prognostic indicator for ischemic stroke. In conclusion, these data highlighted the involvement of the CXCL8-CXCR2 chemotactic axis in the recruitment of granulocytes and NK cells in ischemic stroke. Furthermore, miR-4437 was suggested as a novel target for treating ischemic stroke, while the serum CXCL8 level could be a prognostic factor for ischemic stroke.
Keywords
MRSCXC chemokine receptor 2TNFPE-Cy7PerCPCXCL8CXCR2PBMCsDWIAPCMIRRT-PCRGEOROCFACSPBSDMEMFITCFBSnatural killerAUCDulbecco’s modified Eagle mediumallophycocyaninstandard deviationinterferonIFNInterleukin-8ELISAEnzyme-linked immunosorbent assaydiffusion-weighted imagingcluster of differentiationfluorescence-activated cell sortingfetal bovine serumperipheral blood mononuclear cellsNatural killer cellsIschemic stroketumor necrosis factorPhosphate buffered salinefluorescein isothiocyanateModified Rankin scalearea under the curveMicroRNAwildtypeprognosisGene Expression Omnibusnegative controlGranulocytesreceiver operating characteristic
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Authors
Qianyi He, Xiaojuan Shi, Bin Zhou, Junfang Teng, Chaoqi Zhang, Shasha Liu, Jingyao Lian, Benyan Luo, Guoqiang Zhao, Hong Lu, Yuming Xu, Yajun Lian, Yanjie Jia, Yi Zhang,