CD39 and CD73 in the aortic valve-biochemical and immunohistochemical analysis in valve cell populations and its changes in valve mineralization

Article ID	Journal	Published Year	Pages	File Type
8657639	Cardiovascular Pathology	2018	33 Pages	PDF

Abstract

The presence of ectonucleotidases in valves and especially in aortic valve interstitial cells highlights important local role of purinergic signaling and metabolism. Changes in the local expression and hence the activity of CD39 and CD73 in calcified valves suggest their potential role in CAVD.

Keywords

PBS Erythro-9-(2-hydroxy-3-nonyl) adenine CAVD DMEM EHNA CD39 CD73 FCS AMP ADP HBSS DAPI 4′,6-diamidino-2-phenylindole BSA ECs Dulbecco's modified Eagle's medium Adenosine Adenosine Triphosphate ATP adenosine diphosphate adenosine monophosphate bovine serum albumin calcific aortic valve disease standard error of the mean fetal calf serum Endothelial cells valve Phosphate-buffered saline Hank's balanced salt solution SEM platelet endothelial cell adhesion molecule Nucleotides HPLC high-performance liquid chromatography Calcification

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Health Sciences Medicine and Dentistry Cardiology and Cardiovascular Medicine

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CD39 and CD73 in the aortic valve-biochemical and immunohistochemical analysis in valve cell populations and its changes in valve mineralization

Authors

Ewa Kaniewska-Bednarczuk, Barbara Kutryb-Zajac, Padmini Sarathchandra, Iwona Pelikant-Malecka, Alicja Sielicka, Izabela Piotrowska, Ewa M. Slominska, Adrian H. Chester, Magdi H. Yacoub, Ryszard T. Smolenski,