A high sensitive biosensor based on FePt/CNTs nanocomposite/N-(4-hydroxyphenyl)-3,5-dinitrobenzamide modified carbon paste electrode for simultaneous determination of glutathione and piroxicam

•Electrooxidation of glutathione was studied by modified nanocomposite paste electrode.•This sensor resolved the overlap response of glutathione and piroxicam.•This sensor was used for the determination of above compounds in real samples.

This study describes the development, electrochemical characterization and utilization of novel modified N-(4-hydroxyphenyl)-3,5-dinitrobenzamide-FePt/CNTs carbon paste electrode for the electrocatalytic determination of glutathione (GSH) in the presence of piroxicam (PXM) for the first time. The synthesized nanocomposite was characterized with different methods such as TEM and XRD. The modified electrode exhibited a potent and persistent electron mediating behavior followed by well-separated oxidation peaks of GSH and PXM. The peak currents were linearly dependent on GSH and PXM concentrations in the range of 0.004–340 and 0.5–550 µmol L−1, with detection limits of 1.0 nmol L−1 and 0.1 µmol L−1, respectively. The modified electrode was successfully used for the determination of the analytes in real samples with satisfactory results.