Article ID Journal Published Year Pages File Type
866655 Biosensors and Bioelectronics 2014 7 Pages PDF
Abstract

•Determination of lactate based on hydrogen peroxide amperometric detection is carried out by screen-printed carbon electrodes (SPCE) modified with a film of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA).•The performance of the biosensor in real sweat samples is evaluated.•The long term stability of the biosensors is predicted by a model based on thermal degradation.•The validity of the sweat lactate for the determination of the lactate threshold is discussed.

A novel biosensor for lactate has been developed, using screen-printed carbon electrodes (SPCE) and lactate oxidase (LOx). The active surface of the electrodes was modified using a dispersion of platinum nanoparticle decorated carbon nanofibers (PtNp-CNF) in poly(diallyldimethylammonium) chloride (PDDA) solution. In this way, sensitive, disposable, low cost and reliable hydrogen peroxide sensors were obtained. The immobilisation of LOx on top of these PtNp-CNF-PDDA/SPCEs resulted in amperometric biosensors with high operational stability. The sensitivity of the optimised lactate biosensor was 36.8 (mA/M cm2) with a linear range of 25–1500 µM. The limit of detection was 11 µM (S/N=3). Reproducibility, selectivity and storage stability were also evaluated. Additionally, the stability of the biosensor was also predicted by a model based on thermal degradation. Finally, lactate in sweat and blood samples was determined in a sport test using LOx/PtNp-CNF-PDDA/SPCEs and commercial biosensors respectively. Based on these data, the validity of the sweat lactate for the determination of the lactate threshold is discussed.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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