Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
866771 | Biosensors and Bioelectronics | 2014 | 7 Pages |
•A simple one-pot, monophasic strategy to synthesize PLGNPs was presented.•A novel, lag time-free bioassay to evaluate the activity of phospholipase A2 was developed.•Our new bioassay exhibited high specificity, improved speed and acceptable sensitivity.•An adequate detection limit could be achieved, below the cut-off value of circulating PLA2 in serum.
A facile, monophasic strategy, involving a cooperative solvent, dimethylformamide (DMF), to synthesize core/shell architectured gold–phospholipid hybrid nanoconstructs (PLGNPs) was presented herein. We employed the as-synthesized PLGNPs as enzymatic substrates and detecting probes, leading to the development of a novel, lag time-free quantitative assay to evaluate the activity of Ca2+-dependent phospholipase A2 (PLA2), an inflammatory protein that (i) plays a role in the pathogenesis of many inflammatory diseases, (ii) is a mediator in atherosclerosis and ischemic damage to cardiomyocytes, and (iii) has been implicated in the cause of neurodegenerative diseases. Our new bioassay exhibited high specificity, improved speed (assay time:≤20 min), acceptable sensitivity, and a limit of detection (1.82 nM, equivalent to 0.04 unit/mL and 260 ng/dL) below the cut-off value of circulating PLA2 (2.07 nM, equivalent to 290 ng/dL) present in serum samples collected from healthy testers. We characterized the as-obtained PLGNPs using UV–vis spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS). These PLGNPs were considerably robust and biocompatible—displaying extraordinary stability against salt-induced aggregation, oxidant etching, and repetitive freeze/thaw treatment—because of the presence of their modifying interfacial thiol (1-dodecanethiol) and phospholipid [1,2-dihexadecanoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol)] units.
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