| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 866906 | Biosensors and Bioelectronics | 2013 | 8 Pages |
It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study.
► Selected yEGFP as a reporter. ► Chose and compared the RNR3 and HUG1 promoters as sensors. ► Constructed a yeast septuple mutant with seven genes deleted to enhance the detection sensitivity. ► The resulting biosensors are much more sensitive than previously reported similar systems.
