| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 866915 | Biosensors and Bioelectronics | 2013 | 6 Pages |
A novel turn-on and turn-off sensor based on the fluorescence change of quantum dots (QDs) has been developed to detect the activity of butyrylcholinesterase (BChE). In the turn-on sensing system, we realized the detection of BChE with just one enzyme. A linear calibration plot of the activity of BChE was obtained in the wide amounts range from 10 to 1000 U/L and the detection limit was 10 U/L. In the turn-off sensing system, we realized the sensing of BChE with a wide linear relationship of 10–2000 U/L which was much wider than many other detection methods. We also studied the application in serum sample detection. The BChE sensor shows great performances. Furthermore, the turn-on and turn-off sensing mechanisms were studied extensively. These results showed that our strategy would most probably be applicable in assembling diagnostic micro-device for realizing the rapid clinical analysis of BChE.
► Biosensor was composed of QDs and enzymes, without any complex processes. ► BChE detected by one step enzyme reaction for the first time. ► We studied the detection mechanisms of turn-on and turn-off detection system. ► We demonstrated the application in real serum samples. ► This work presents a feasible approach for detecting some kinds of substrate.
