Detecting Lyme disease using antibody-functionalized single-walled carbon nanotube transistors

We examined the potential of antibody-functionalized single-walled carbon nanotube (SWNT) field-effect transistors (FETs) to use as a fast and accurate sensor for a Lyme disease antigen. Biosensors were fabricated on oxidized silicon wafers using chemical vapor deposition grown carbon nanotubes that were functionalized using diazonium salts. Attachment of Borrelia burgdorferi (Lyme) flagellar antibodies to the nanotubes was verified by atomic force microscopy and electronic measurements. A reproducible shift in the turn-off voltage of the semiconducting SWNT FETs was seen upon incubation with B. burgdorferi flagellar antigen, indicative of the nanotube FET being locally gated by the residues of flagellar protein bound to the antibody. This sensor effectively detected antigen in buffer at concentrations as low as 1 ng/ml, and the response varied strongly over a concentration range coinciding with levels of clinical interest. Generalizable binding chemistry gives this biosensing platform the potential to be expanded to monitor other relevant antigens, enabling a multiple vector sensor for Lyme disease. The speed and sensitivity of this biosensor make it an ideal candidate for development as a medical diagnostic test.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Functionalized carbon nanotube transistors used to detect Lyme disease antigen. ► Biosensors based on commercial monoclonal antibodies to Lyme flagellar protein. ► Antibody binding chemistry verified by Atomic Force Microscopy and electronic measurements. ► Detection limit for Lyme flagellar protein was 1 ng/mL with measurement time of 20 min. ► General protein binding chemistry implies the approach could be used for any antigen.