An integrated solution for rapid biosensing with robust linker free covalent bindingsurfaces

A novel integrated biosensor methodology is proposed and demonstrated. The methodology utilizes a nitrogen-containing plasma polymer to achieve linker-free binding of a layer of biorecognition molecules. The sensor surface has been shown to maintain its performance after freeze-drying providing a long shelf life under ambient conditions. The sensor is configured for single wavelength ellipsometric detection providing a low cost, versatile, and rapid sensing and diagnosis platform suitable for a wide range of applications and end-users. The merits of the methodology are demonstrated using three antigen–antibodypairs.

► An integrated solution of a novel robust and end-user ready biosensor method using ellipsometric detection is shown. ► We developed linker-free protein covalent binding surfaces for the biosensors. ► Optimized optical design of the sensors is demonstrated. ► Freeze-dry method was used to the biosensors prior to storage at ambient temperature. ► merits of the methodology were demonstrated using three antigen–antibodypairs.