| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 867256 | Biosensors and Bioelectronics | 2013 | 6 Pages |
A Fiber-Optic Localized Surface Plasmon Resonance (FO LSPR) sensor was fabricated using spherical gold nanoparticles (Au NPs) on a flattened end-face of the optical fiber. The Au NPs were easily synthesized by the Turkevich method and were immobilized on the end-face of the optical fiber by using a self-assembled monolayer (SAM). In order to examine the possibility of its application as a biosensor for label-free immunoassays, the fabricated FO LSPR sensor was used for the detection of the antibody–antigen reaction of interferon-gamma (IFN-γ) and the limit of detection (LOD) was approximately 2 pg/ml. Herein, The antibodies and bovine serum albumins (BSAs) were immobilized on the Au NPs by physisorption. Also, the FO LSPR sensor was used for the detection of a prostate-specific antigen (PSA) and the LOD was 1 pg/ml below. The fabricated FO LSPR sensor can be used for real-time label-free immunoassay having fast detection time, high resolution and sensitivity. In addition, the proposed sensor platform has the advantages of low cost, simple optical setup, remote sensing, simple fabrication, real-time detection, low sample volume, and potential application to in-vivo detection systems.
► A biosensor was developed by using a Fiber-Optic Localized Surface Plasmon Resonance. ► The sensor can rapidly detect the antibody–antigen reaction of an interferon-gamma. ► It also can detect a prostate-specific antigen with high resolution and sensitivity. ► The biosensor can be applied for clinical testing.
