Highly sensitive and selective uric acid biosensor based on RF sputtered NiO thin film

Present study highlights the importance of RF sputtered NiO thin film deposited on platinum coated glass substrate (NiO/Pt/Ti/glass) as a potential matrix for the realization of highly sensitive and selective uric acid biosensor. Uricase has been immobilized successfully onto the surface of NiO matrix by physical adsorption technique. The prepared bioelectrode (uricase/NiO/Pt/Ti/glass) is utilized for sensing uric acid using the cyclic voltammetry and UV visible spectroscopy techniques. The bioelectrode is found to exhibit highly efficient sensing response characteristics with high sensitivity of 1278.48 μA/mM; good linearity of 0.05–1.0 mM, and very low Michaelis–Menten constant (km) of 0.17 mM indicating high affinity of uricase towards the analyte. The enhanced response is due to the development of NiO matrix with good electron transport property and nanoporous morphology for effective loading of enzyme with preferred orientation.

► In this study we prepared Uric acid biosensor based on RF sputtered NiO thin film matrix. ► Efficient detection of Uric acid and towards the realization of hand-held bio chip. ► Highly selective towards the detection of uric acid in presence of various interferents. ► The prepared bioelectrode exhibits fast response time of 5 s and high enzyme activity.