Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
867757 | Biosensors and Bioelectronics | 2011 | 5 Pages |
Abstract
We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift can be detected as a color change in the darkfield image of the gold nanoparticles. Parallel detection of hundreds of gold nanoparticles with a calibrated true color camera enabled us to detect specific binding of target DNA. This enables a limit of detection below 1.0 × 10−14 M without the need for a spectrometer or a scanning stage.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Remco Verdoold, Ron Gill, Felicia Ungureanu, Robert Molenaar, Rob P.H. Kooyman,