Selective collection and detection of leukemia cells on a magnet-quartz crystal microbalance system using aptamer-conjugated magnetic beads

A novel method for selective collection and detection of human acute leukemia cells has been proposed using aptamer-conjugated magnetic beads (apt-MBs) and a magnet-quartz crystal microbalance (QCM) system. The sgc8c aptamer-conjugated MBs specifically binding to CCRF-CEM cells were used for target cell extraction from complex matrixes, and the magnet–QCM system was successfully applied for quantitative cell detection, requiring no further labeling of cells. The accumulation of MBs-conjugated CCRF-CEM cells on a quartz crystal gold electrode surface under a magnetic field resulted in decreased resonant frequency. A linear relationship between the frequency shift and cell concentration over the range of 1 × 104–1.5 × 105 cells mL−1 was obtained, with a detection limit of 8 × 103 cells mL−1. The applicability of the method for target cell detection from cell mixture was satisfactory.