Article ID Journal Published Year Pages File Type
867860 Biosensors and Bioelectronics 2010 7 Pages PDF
Abstract

A novel catechol biosensor was described based on the immobilization of polyphenol oxidase (PPO) into polyaniline (PANI), which was easily constructed by direct electropolymerization of aniline in a solution containing ionic liquid, 1-ethyl-3-methylimidazolium ethyl sulfate (EMIES). The developed biosensor for the detection of catechol has a linear range of 1.25–150 μmol dm−3. The maximum response current (Imax) and the Michaelis–Menten constant (k′m)(k′m) are 0.62 μA and 146 μmol dm−3, respectively. The activation energy (Ea) of the PPO catalytic reaction is 31.1 kJ mol−1 in the B–R buffer. The biosensor shows good reproducibility (a relative standard deviation of 3.1% was obtained) and remarkable long-term stability (it retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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