Adding to the complexity of fetal and neonatal alloimmune thrombocytopenia: Reduced fibrinogen binding in the presence of anti-HPA-1a antibody and hypo-responsive neonatal platelets

Article ID	Journal	Published Year	Pages	File Type
8679681	Thrombosis Research	2018	8 Pages	PDF

Abstract

Reduced fibrinogen binding in the presence of anti-HPA-1a antibodies may disturb the neonatal hemostatic balance, characterized by poorly responsive platelets. This effect may operate in parallel to platelet destruction and contribute to the clinical outcome in FNAIT.

Keywords

Isoantibodies ADP TRAP APC CFT MPV vWF RT-PCR MESF PAR1 SSC MCF FSC HEPES MFI FITC IgG PAC-1 PC7 FNAIT PBS 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid AUC BSA cpd adenosine diphosphate Blood coagulation tests bovine serum albumin allophycocyanin human platelet antigen Human platelet antigens EDTA Ethylenediaminetetraacetic acid immunoglobulin G analysis of variance ANOVA Fetal and neonatal alloimmune thrombocytopenia Neonatal alloimmune thrombocytopenia Maximum clot firmness cluster of differentiation Intracranial hemorrhage Room temperature Clot formation time Clotting time median fluorescence intensity Von Willebrand factor Phosphate buffered saline fluorescein isothiocyanate Fibrinogen mean platelet volume ICH area under the curve molecules of equivalent soluble fluorochrome HPA arbitrary units real-time polymerase chain reaction forward scatter sideward scatter Blood platelets Glycoprotein Protease-activated receptor 1