Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
868055 | Biosensors and Bioelectronics | 2010 | 6 Pages |
A colorimetric, label-free, and nonaggregation-based gold nanoparticles probe has been developed for the detection of melamine. Gold nanoparticles were generated using 3,5-dihydroxybenzoic acid as a reducer without adding gold nanoparticle seeds at room temperature. Upon the addition of melamine, the reducer 3,5-dihydroxybenzoic acid can interact with melamine through strong hydrogen-bonding interaction. Consequently, the formation of gold nanoparticles was interrupted by melamine since there was not enough reducer for the reduction of Au3+ ion. And the color change from purple to yellowgreen with increasing melamine concentration was observed. The plasmon absorbance of the formed Au-NPs allows the quantitative detection of melamine. A sensitive linear correlation existed between the absorbance and the logarithm of melamine concentration ranging from 1 × 10−9 M to 1 × 10−5 M with a linear coefficiency of 0.993. The system has a high selectivity to melamine with a low detection limit of 8 × 10−10 M.