| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 868457 | Biosensors and Bioelectronics | 2010 | 7 Pages |
Abstract
An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.
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Authors
Marie Trévisan, Manfred Schawaller, Gerald Quapil, Eliane Souteyrand, Yves Mérieux, Jean-Pierre Cloarec,