| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 868710 | Biosensors and Bioelectronics | 2009 | 5 Pages |
Abstract
We report experimental results concerning the detection of 2,4-dinitrophenol, under its free form or coupled to human serum albumin using Fourier transform infrared spectroscopy-based sensors. Competitive immunoreactions were carried out using several anti-dinitrophenol monoclonal antibodies. Comparison with enzyme-linked immunosorbent assays in competition is given for standard operating conditions. FTIR detection limits are comparable to those obtained by ELISA. The limits of detection are about 5–15 ng/mL for the coupled DNP. Using the LO-DNP61 antibody, a detection limit of ≅ 5 ng/mL was also estimated for the free DNP molecules but is much higher for the other antibodies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
E. Gosselin, M. Gorez, M. Voué, O. Denis, J. Conti, N. Popovic, A. Van Cauwenberge, E. Noel, J. De Coninck,