Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
868982 | Biosensors and Bioelectronics | 2008 | 8 Pages |
Abstract
The direct electrically addressable deposition of diazonium-modified antibodies is examined for electrochemical immunosensing applications. The immobilized antibodies can be detected by the use of electroactive enzyme tags and nanoparticle-gold labeling. Control over antibody functionalization density and minimal spontaneous grafting of diazonium–antibody adducts is shown. The utility of the technique for a sandwich immunoassay as well as the ability to individually and selectively address closely spaced microelectrodes for multi-target protein detection in an array format is demonstrated.
Keywords
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ronen Polsky, Jason C. Harper, David R. Wheeler, Shawn M. Dirk, Dulce C. Arango, Susan M. Brozik,