| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 869108 | Biosensors and Bioelectronics | 2007 | 5 Pages |
The hydroxamate-type artificial siderophore, tris[2-{3-(N-acetyl-N-hydroxamino)propylamido}propyl]aminomethane (TAPPA) and its FeIII complex, FeIII-TAPPA were prepared and characterized by several spectroscopic methods. FeIII-TAPPA exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, Microbacterium flavescens, suggesting that FeIII-TAPPA can permeate the cell membrane of the microorganism. The adsorption of the FeIII-siderophore complex onto a deposited Au substrate was achieved by a stepwise self-assembling method. The modification of FeIII-TAPPA on the surface was confirmed from the cyclic voltammogram of the resultant Au electrode, FeIII-TAPPA/Au. The adsorption experiments of M. flavescens with FeIII-TAPPA/Au were monitored by optical, scanning electron, and atomic force microscopy and quartz crystal microbalance (QCM) measurements. These results clearly indicate that FeIII-TAPPA/Au can immobilize M. flavescens. This adsorption characteristic is due to the interaction between FeIII-TAPPA on an Au electrode and a receptor/binding protein within the cell membrane.
