Article ID Journal Published Year Pages File Type
869323 Biosensors and Bioelectronics 2010 6 Pages PDF
Abstract

Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H2O2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5 h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE5 h (5 h deposition time) exhibited remarkable sensitivity (45.86 μA mM−1 cm−2) towards H2O2 with a detection limit of 0.42 μM (S/N = 3), linearity up to 15 mM and a response time of 4 s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
, , , , ,