| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 869362 | Biosensors and Bioelectronics | 2010 | 5 Pages |
An optical fiber nanobiosensor was constructed to successfully detect a general cancer biomarker, telomerase at single cell level with its nanoscale tip. The nanotip immobilized with a specific antibody was inserted into a MCF-7 breast cancer cell nucleus to capture telomerases directly, after which an in vitro enzymatic sandwich immunoassay was performed to achieve sensitive single living cell detection. The nanotip inserted into MCF-7 cell nucleus provides significantly higher average (F − F0)/F0 ratio than that of human mesenchymal stem cell (hMSC) nucleus, demonstrating the successful detection of the telomerase over-expression in cancer cells as compared to normal cells. The detection in the cytoplasm shows much smaller average ratio than that in the nucleus of MCF-7 cells while clearly verifies the nuclear localization of telomerase. The successful detection of telomerase over-expression in a single living cell for the first time may provide a potential method for cancer detection, and also demonstrate a universal approach that can be used to detect other low expression proteins in a single living cell.
