| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 869549 | Biosensors and Bioelectronics | 2008 | 4 Pages |
Abstract
To amplify the heat-signal generated by MIP catalyzed solvolysis of phenylacetate the reaction has been combined for the first time in a reactor with the subsequent oxidation by immobilized tyrosinase. The polymer cleaves the substrate and the released phenol is afterwards converted to o-benzoquinone by the tyrosinase. The separated and sequentially coupled reactions are characterized by the heat generated in a thermistor. The sequential substrate conversion results in a combined heat generation which results a five times higher signal than compared to the polymer alone.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
K. Lettau, M. Katterle, A. Warsinke, F.W. Scheller,