Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
870107 | Biosensors and Bioelectronics | 2007 | 8 Pages |
Abstract
We demonstrate the microfabrication of a low-noise silicon based device with integrated silver/silver chloride electrodes used for the measurement of single ion channel proteins. An aperture of 150 μm diameter was etched in a silicon substrate using a deep silicon reactive ion etcher and passivated with 30 nm of polytetrafluoroethylene via chemical vapor deposition. The average recorded noise in measurements of lipid bilayers was reduced by a factor of four through patterning of a 75 μm thick SU-8 layer around the aperture. Integrated electrodes were fabricated on both sides of the device and used for repeatable, stable, giga-seal bilayer formations as well as characteristic measurements of the transmembrane protein OmpF porin.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
S.J. Wilk, L. Petrossian, M. Goryll, T.J. Thornton, S.M. Goodnick, J.M. Tang, R.S. Eisenberg,