| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 870407 | Biosensors and Bioelectronics | 2006 | 9 Pages |
Abstract
In this work, the results of a method based on an electrochemical biosensor to detect DNA damage in vitro for the evaluation of the antioxidant properties of plant extracts are reported. The biosensor consisted of a dsDNA immobilized on a screen-printed electrode surface (SPE). DNA damage was promoted by the generation of the OH radicals via Fenton-type reaction. The interaction of the radical species with immobilised DNA in the absence and presence of antioxidants was evaluated by means of changes in the guanine oxidation peak obtained by square wave voltammetry. The results demonstrated that the DNA-based biosensor is suitable as a rapid screening test for the evaluation of antioxidant properties of samples.
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Authors
Lucilene Dornelles Mello, Silvia Hernandez, Giovanna Marrazza, Marco Mascini, Lauro Tatsuo Kubota,