Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8724458 | Revista Argentina de Endocrinología y Metabolismo | 2016 | 7 Pages |
Abstract
Immunochemical characterisation of ZnT8A present in type 1 Argentinian diabetic patients was accomplished, employing different variants of ZnT8. An increased detection of autoimmunity was found when ZnT8A was employed in combination with the other islet-autoantibodies. The presence of autoantibodies that recognized only constructions containing Arg reveals the existence of epitopes dependent of the amino acid present at residue 325. Furthermore, application of dimeric constructions revealed the existence of quaternary structure-defined epitopes which were recognized by type 1 diabetic patients. Finally, the highest combined sensitivity and specificity for routine screening of ZnT8A was accomplished with the heterodimeric construction.
Keywords
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Endocrinology, Diabetes and Metabolism
Authors
Natalia Inés Faccinetti, Bruno David Rovitto, Luciano Lucas Guerra, Adriana Victoria Sabljic, Ruben Francisco Iacono, Liliana Trifone, Edgardo Poskus, Aldana Trabucchi, Silvina Noemà Valdez,