Ameliorating effects of Nigella sativa oil on aggravation of inflammation, oxidative stress and cytotoxicity induced by smokeless tobacco extract in an allergic asthma model in Wistar rats

Article ID	Journal	Published Year	Pages	File Type
8735906	Allergologia et Immunopathologia	2018	10 Pages	PDF

Abstract

Our data have proven that severe concurrent exposure to allergen and ST increases airway inflammation and oxidative stress in previously sensitized rats. They also suggest that the oral NSO treatment could be a promising treatment for asthma.

Keywords

Nigella sativa TBARS NBT LPO DTNB GPX GSH IL-4 OVA b.w.DNPH TBA CAT IgE IFN-γ NSO LD50 Th1 Th2 STE AHR PBS NPSH MDA smokeless tobacco extract 2,4-dinitrophenylhydrazine ROS Experimental asthma Thiobarbituric acid Airway inflammation Ovalbumin Immunoglobulin E interleukin-4 BALF nitro blue tetrazolium Enzyme-linked immunosorbent assay ELISA Oxidative stress smokeless tobacco Thymoquinone standard error mean Nigella sativa oil SOD Superoxide dismutase malondialdehyde Bronchoalveolar lavage fluid phosphate buffer solution SEM Wistar rats median lethal dose Nitric oxide body weight Airway hyperresponsiveness Lipid peroxidation Non-protein thiols Catalase reduced glutathione Interferon gamma glutathione peroxidase Reactive oxygen species