Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8737581 | Human Immunology | 2018 | 27 Pages |
Abstract
Noninvasive methods for the early diagnosis of chronic antibody-mediated rejection (cAMR) are desired for patients with de novo (dn) donor-specific HLA antibody (DSA). This study aimed to elucidate the clinical relevance of immune-related gene expression in peripheral blood of kidney transplant recipients. The expression levels of fourteen key molecules (Foxp3, CTLA-4, CCR7, TGF-β, IGLL-1, IL-10, ITCH, CBLB, Bcl-6, CXCR5, granzyme B, CIITA, Baff, TOAG-1/TCAIM) related to regulatory/cytotoxic function of immune cells were compared in 93 patients, which were divided into Groups A (clinical cAMR with dn DSA, nâ¯=â¯16), B (subclinical cAMR with dn DSA, nâ¯=â¯17), C (negative cAMR with dn DSA, nâ¯=â¯21) and D (stable function without dn DSA, nâ¯=â¯39). CIITA mRNA expression levels in groups B and C were significantly lower than those in group D (pâ¯<â¯0.01). Moreover, the CTLA-4 mRNA expression in group A was significantly higher than that in groups B and C (pâ¯<â¯0.01). ROC curve analysis suggested that CIITA (AUCâ¯=â¯0.902) and CTLA-4 (AUCâ¯=â¯0.785) may serve as valuable biomarkers of the stage of dn DSA production and clinical cAMR, respectively. In addition to dn DSA screening, monitoring of CIITA and CTLA-4 in peripheral blood could offer useful information on the time course of the development of cAMR.
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Authors
Takayuki Yamamoto, Kenta Iwasaki, Kenta Murotani, Asami Takeda, Kenta Futamura, Manabu Okada, Makoto Tsujita, Takahisa Hiramitsu, Norihiko Goto, Shunji Narumi, Yoshihiko Watarai, Kunio Morozumi, Kazuharu Uchida, Takaaki Kobayashi,