Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8737675 | Human Immunology | 2018 | 10 Pages |
Abstract
For over 50â¯years, the International HLA and Immunogenetics Workshops (IHIW) have advanced the fields of histocompatibility and immunogenetics (H&I) via community sharing of technology, experience and reagents, and the establishment of ongoing collaborative projects. Held in the fall of 2017, the 17th IHIW focused on the application of next generation sequencing (NGS) technologies for clinical and research goals in the H&I fields. NGS technologies have the potential to allow dramatic insights and advances in these fields, but the scope and sheer quantity of data associated with NGS raise challenges for their analysis, collection, exchange and storage. The 17th IHIW adopted a centralized approach to these issues, and we developed the tools, services and systems to create an effective system for capturing and managing these NGS data. We worked with NGS platform and software developers to define a set of distinct but equivalent NGS typing reports that record NGS data in a uniform fashion. The 17th IHIW database applied our standards, tools and services to collect, validate and store those structured, multi-platform data in an automated fashion. We have created community resources to enable exploration of the vast store of curated sequence and allele-name data in the IPD-IMGT/HLA Database, with the goal of creating a long-term community resource that integrates these curated data with new NGS sequence and polymorphism data, for advanced analyses and applications.
Keywords
SBTSFTPIUPACIMGTGFEHMLIPDSSPGenotype ListKirSSOsequence-specific primingcomma-separated valuesNGSrSCASequence-SpecificCSVHuman leukocyte antigenHLAInternational Union of Pure and Applied ChemistryXMLImmunogeneticsDatabasesequence-based typingReference Strand Conformation AnalysisNext generation sequencingextensible markup languagePrincipal investigatorData managementWorkshopkiller-cell immunoglobulin-like receptor
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Immunology
Authors
Chia-Jung Chang, Kazutoyo Osoegawa, Robert P. Milius, Martin Maiers, Wenzhong Xiao, Marcelo Fernandez-ViÅa, Steven J. Mack,