Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8740292 | Journal of Hospital Infection | 2017 | 27 Pages |
Abstract
The Check-Direct ESBL Screen for the BD MAX is an easy-to-perform, quick molecular diagnostic test with the potential to significantly speed up screening for rectal ESBL-E carriage. Discrepancies were observed between the culture-based protocol and the qPCR in 4.5% of tested samples. Existing challenges for implementing qPCR are its limited sensitivity, the need for thorough knowledge of the local ESBL-E genes, and interpretation of culture-negative but qPCR-positive samples. It is believed that the limited sensitivity of qPCR could be optimized by including blaTEM as a molecular target, and improving the limit of detection.
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Authors
T. Engel, B.J. Slotboom, N. van Maarseveen, A.A. van Zwet, M.H. Nabuurs-Franssen, F. Hagen,