Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8743762 | Transplant Immunology | 2018 | 5 Pages |
Abstract
Preformed anti-human leukocyte antigen (HLA) antibodies may be present in the blood of kidney transplant candidates. The production of these antibodies may occur in the post-transplant period, with the possible development of donor-specific antibodies (DSA). Luminex-based tests, such as the single antigen (SA) assay and the Luminex crossmatch (Xm-DSA) assay are the most commonly used tools to detect anti-HLA antibodies, due to their high sensitivity and specificity. This cross-sectional study aimed to compare the findings of two methods for the detection of DSAs after kidney transplant: SA and Xm-DSA. A total of 122 patients who underwent deceased donor kidney transplant at Hospital de ClÃnicas de Porto Alegre were included. The SA assay detected anti-class I HLA DSAs in 17 patients (13.9%) and anti-class II HLA DSAs in 22 patients (19.6%), whereas the Xm-DSA detected DSAs in 18 patients (14.8%) both against class I and class II antigens. There was agreement between the two methods for class I (kappaâ¯=â¯0.66, pâ¯=â¯0.001) and class II (kappaâ¯=â¯0.54, pâ¯=â¯0.025) antigens. The incidence of DSAs as obtained by the SA assay was 15.57%, and the most prevalent DSAs were those against HLA-DR antigens. Patient survival at 3â¯years was 92%. The two techniques assessed in this study provide important information on the presence of DSAs and may help in the post-transplant patient monitoring and in immunosuppressive strategy.
Keywords
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Immunology
Authors
Beatriz Chamun Gil, Adriane Stefani Silva Kulzer, Priscila de Moraes, Realdete Toresan, Alessandra da Rosa Vicari, Iara dos Santos Fagundes, Jóice Merzoni, Gisele Menezes Ewald, Jacqueline Moraes Cardone, Fernanda Gamio Silva, Roberto Ceratti Manfro,