Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8746960 | Journal of Virological Methods | 2018 | 21 Pages |
Abstract
Fowlpox lesions were sampled on hens, total DNA was extracted and processed for sequencing on a MinION, Oxford Nanopore. We readily generated whole APV genomes from cutaneous or tracheal lesions, without any isolation or PCR-based enrichment: Fowlpox virus reads loads ranged from 0.75% to 2.62% and reads up to 61â¯kbp were generated and readily assembled into 3 APV complete genomes. This long read size eases the assembly step and lowers the bioinformatics capacity requirements and processing time compared to huge sets of short reads. The complete genome analysis confirmed that these Fowlpox viruses cluster within clade A1 and host full length reticuloendotheliovirus (REV) inserts. The pathobiological relevance of REV insert, although a classical feature of fowlpoxviruses (FPVs), should be further investigated. Surveillance of emerging poxviruses could greatly benefit from real-time whole genome sequencing.
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Authors
Guillaume Croville, Guillaume Le Loc'h, Catherine Zanchetta, Maxime Manno, Christelle Camus-Bouclainville, Christophe Klopp, Maxence Delverdier, Marie-Noëlle Lucas, Cécile Donnadieu, Mattias Delpont, Jean-Luc Guérin,