Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8746965 | Journal of Virological Methods | 2018 | 18 Pages |
Abstract
Recently, a novel duck adenovirus (designated as duck adenovirus 3, DAdV-3) was discovered in Muscovy ducks, China. Here, we developed a TaqMan-based real-time PCR assay (qPCR) for the detection of DAdV-3 infection. After the optimization of the qPCR conditions, the lower limit of detection for DAdV-3 infection was 40.9 copies/μl. No cross-reactivity was observed with other duck-derived pathogens. Intra- and inter-assay variability was less than 2.30%. DAdV-3 was detected in embryos and newly hatched ducklings by qPCR assay, the findings provided evidence of possible vertical transmission of DAdV-3. The developed qPCR analysis showed high specificity, sensitivity, and reproducibility, thereby indicating that it can be used in future investigations on the pathogenesis and epidemiology of DAdV-3.
Keywords
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Virology
Authors
Chunhe Wan, Cuiteng Chen, Longfei Cheng, Guanghua Fu, Shaohua Shi, Rongchang Liu, Hongmei Chen, Qiuling Fu, Yu Huang,