Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8746982 | Journal of Virological Methods | 2018 | 7 Pages |
Abstract
A licensed vaccine for respiratory syncytial virus (RSV) has yet to be developed, and a reliable and repeatable neutralizing assay is indispensable for vaccine development. Here, we demonstrated an optimized high-throughput RSV neutralization assay that utilizes a fluorescence plate reader (reader) as a substitute for flow cytometry to detect fluorescent signals in RSV-A2 mKate-infected cells. Furthermore, this study tested the influence of virus input and infectivity on the neutralizing assay and highlighted critical factors (together with a suggested protocol) for obtaining stable data using this assay.
Keywords
Related Topics
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Immunology and Microbiology
Virology
Authors
Yong-Peng Sun, Wei Zhang, Qin-Jian Zhao, Jian-Li Cao, Lu-Jing Zhang, Jiang-Yan Xiang, Jun-Yu Si, Xue Lin, Li Chen, Zi-Zheng Zheng, Ning-Shao Xia,