Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8747039 | Journal of Virological Methods | 2018 | 31 Pages |
Abstract
Dengue virus (DENV) infections elicit antibody responses to the non-structural protein 1 (NS1) that are associated with protection against disease. However, the antibody isotypes and subclasses involved, and their kinetics have not been extensively studied. We characterized the antibody responses to DENV NS1 by enzyme-linked immunosorbent assay (ELISA) in a longitudinal cohort of 266 confirmed dengue cases in Recife, Northeast Brazil. Samples were collected during the febrile phase and up to over 3 years after onset of symptoms. The antibodies investigated [IgA, IgM, total IgG (all subclasses measured together) and each subclass (IgG2, IgG3 and IgG4) measured separately] had distinct kinetic profiles following primary or secondary DENV infections. Of interest, most of these antibodies were consistently detected greater than 6 months after onset of symptoms, except for IgG3. Anti-dengue NS1-specific IgG was consistently detected from the acute phase to beyond 3 years after symptom onset. In contrast, anti-dengue NS1-specific IgG3 was detected within the first week, peaked at week 2-3, and disappeared within 4-6 months after onset of symptoms. The mean duration of the IgG3 positive signal was 149 days (ranging from 126 to 172 days). In conclusion, anti-dengue NS1-specific IgG and IgG3 are potential biomarkers of long-term and recent (less than 6 months) DENV infections, respectively.
Keywords
DHFCYD-TDVIQCIgG3PRNTZIKVWNVUSUVNS1TBEvDENVGMCIVIgDFCIgGROCLLOQEC50plaque reduction neutralization testimmunoglobulin GBiomarkerDengue hemorrhagic feverDengue feverELISAEnzyme-linked immunosorbent assayJEVLong termrecent infectionlower limit of quantitationGeometric mean concentrationhalf maximal effective concentrationpolymerase chain reactionPCRUsutu virusTick-borne encephalitis virusJapanese encephalitis virusDengue virusZika virusWest Nile virusAntibodyIgG subclassesinternal quality controlreceiver operating characteristic
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Authors
Eduardo J.M. Nascimento, James W. Huleatt, Marli T. Cordeiro, Priscila M.S. Castanha, James K. George, Eduard Grebe, Alex Welte, Monique Brown, Donald S. Burke, Ernesto T.A. Marques,