Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751410 | Virology | 2018 | 8 Pages |
Abstract
Pseudorabies virus (PRV), the etiological pathogen of Aujeszky's disease, belongs to the Alphaherpesvirus subfamily. Large latency transcript (LLT), the most abundant PRV transcript, harbors a ~ 4.6â¯kb microRNA (miRNA) cluster-encoding intron. To investigate the function of the LLT miRNA cluster during the life cycle of PRV, we generated a miRNA cluster mutation virus (PRV-âmiR cluster) and revertant virus. Analysis of the growth kinetics of PRV-ÎmiR cluster-infected cells revealed significantly smaller plaques and lower titers than the wild-type and revertant viruses. The mutation virus exhibited increased IE180 and decreased EP0 expression. The clinical symptoms observed in mice infected with PRV-ÎmiR cluster revealed that the miRNA cluster is involved in the pathogenesis of PRV. Physical parameters, virus shedding assays, and the SN50 titers revealed that the miRNA cluster enhances PRV virulence in pigs. Collectively, our findings suggest that the full-length miRNA cluster is involved in PRV replication and virulence.
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Immunology and Microbiology
Virology
Authors
Xin Wang, Mei-Mei Zhang, Kai Yan, Qi Tang, Yi-Quan Wu, Wen-Bo He, Huan-Chun Chen, Zheng-Fei Liu,