Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751437 | Virology | 2018 | 10 Pages |
Abstract
Classical swine fever virus (CSFV) C-strain was developed through hundreds of passages of a highly virulent CSFV in rabbits. To investigate the molecular basis for the adaptation of C-strain to the rabbit (ACR), a panel of chimeric viruses with the exchange of glycoproteins Erns, E1, and/or E2 between C-strain and the highly virulent Shimen strain and a number of mutant viruses with different amino acid substitutions in E2 protein were generated and evaluated in rabbits. Our results demonstrate that Shimen-based chimeras expressing Erns-E1-E2, Erns-E2 or E1-E2 but not Erns-E1, Erns, E1, or E2 of C-strain can replicate in rabbits, indicating that E2 in combination with either Erns or E1 confers the ACR. Notably, E2 and the amino acids P108 and T109 in Domain I of E2 are critical in ACR. Collectively, our data indicate that E2 is crucial in mediating the ACR, which requires synergistic contribution of Erns or E1.
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Authors
Yongfeng Li, Libao Xie, Lingkai Zhang, Xiao Wang, Chao Li, Yuying Han, Shouping Hu, Yuan Sun, Su Li, Yuzi Luo, Lihong Liu, Muhammad Munir, Hua-Ji Qiu,