Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751439 | Virology | 2018 | 6 Pages |
Abstract
goose parvovirus (GPV) belongs to the Dependoparvovirus genus in Parvovirinae subfamily within Parvoviridae family, is the etiological agent of Derzsy's disease. Nuclear localization signal (NLS) is important for parvovirus lifecycle in the delivery of genomes and the structural protein of progeny virus into the nucleus. Here, NLS was first identified in GPV. By using the PSORT II program, a basic region (BR, 160YPVVKKPKLTEE171) in the N-terminus of VP1 was found, which predicted as putative NLS motif of goose parvovirus capsid. The GPV BR could transfer both small reporter proteins (EGFP) and large reporter protein (β-galactosidase) into the nucleus by Immunofluorescence assay. Furthermore, the K164A, or K165A, or K167A substitutions mutation of GPV VP1 did abolish its nuclear localization, suggesting that the 164â¯K, 165â¯K and 167â¯K residues in the 160YPVVKKPKLTEE171 are required for its for nuclear import. Our finding may help us to gain a better understand of GPV lifecycle.
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Authors
Shun Chen, Peng Liu, Yu He, Chao Yang, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Qiao Yang, Ying Wu, Anchun Cheng,