Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751562 | Virology | 2018 | 8 Pages |
Abstract
Homing endonucleases are a group of site-specific endonucleases that initiate homing, a nonreciprocal transfer of its own gene into a new allele lacking this gene. This work describes a novel phage T4 endonuclease, SegD, which is homologous to the GIY-YIG family of homing endonucleases. Like other T4 homing endonucleases SegD recognizes an extended, 16Â bp long, site, cleaves it asymmetrically to form 3â²-protruding ends and digests both unmodified DNA and modified T-even phage DNA with similar efficiencies. Surprisingly, we revealed that SegD cleavage site was identical in the genomes of segD- and segD+ phages. We found that segD gene was expressed during the T4 developmental cycle. Nevertheless, endonuclease SegD was not able to initiate homing of its own gene as well as genetic recombination between phages in its site inserted into the rII locus.
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Andrey S. Sokolov, Oleg R. Latypov, Peter M. Kolosov, Michael G. Shlyapnikov, Tamara A. Bezlepkina, Natalia S. Kholod, Farid A. Kadyrov, Igor E. Granovsky,