Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751635 | Virus Research | 2018 | 5 Pages |
Abstract
Recombinant foot-and-mouth disease virus-like particles (VLPs) can be expressed in a number of expression systems including plants. However, yields in plants have formerly been shown to be low, possibly due to their acid and/or heat lability, previously shown to affect VLP yields produced in other systems. This work describes the introduction of mutations into the FMDV structural protein-encoding gene (P1-2A) which have been previously shown to increase acid and thermostability. VLPs expressed in plants using the mutant constructs had negative rather than positive effects on yield and temperature and acid stability compared to the control.
Keywords
Related Topics
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Immunology and Microbiology
Virology
Authors
Varusha Pillay Veerapen, Albertha R. van Zyl, Edward P. Rybicki, Ann E. Meyers,